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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Innate immune activation of astrocytes impairs neurodevelopment via upregulation of follistatin-like 1 and interferon-induced transmembrane protein 3

Fig. 3

Effect of Fstl1 knockdown in astrocytes on polyI:C ACM-induced impairment of neuronal development. Astrocytes were transfected with control siRNA (CON), Fstl1 siRNA #1 (#1), or Fstl1 siRNA #2 (#2) before polyI:C or vehicle treatment, and then ACM and cell lysate samples were prepared 24 h after polyI:C treatment. a Representative western blot images of Fstl1 protein levels in ACM derived from siRNA-treated astrocytes. Values indicate the means ± SE (n = 4). **p < 0.01 versus control ACM, ##p < 0.01 versus control siRNA-treated polyI:C ACM. b Effect of polyI:C ACM derived from Fstl1 knockdown astrocytes on MAP2-positive dendrite length of primary cultured neurons (DIV7). Neurons were cultured for 5 days (DIV2-7) with control ACM or polyI:C ACM derived from astrocytes transfected with control siRNA (CON) or Fstl1 siRNA (#1 or #2). Values indicate the means ± SE of more than three independent experiments (n = 28–59 neurons). **p < 0.01 versus control ACM, ##p < 0.01 versus control siRNA-treated polyI:C ACM. Scale bar, 50 μm

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