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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Complement-dependent bystander injury to neurons in AQP4-IgG seropositive neuromyelitis optica

Fig. 3

Evidence for a complement bystander mechanism for AQP4-IgG/complement-induced neuron injury in astrocyte-neuron cocultures. a C5b-9 immunofluorescence (green) in astrocyte-neuron cocultures with dead cell stain (red) at 2 h after incubation with 20 μg/ml AQP4-IgG and 2% human complement, immunostained for GFAP or MAP2 (gray). b C5b-9 and C1q immunofluorescence of cocultures with cell markers GFAP and MAP2 treated as in a. Filled arrowheads indicate C5b-9 or C1q on astrocytes, and open arrowheads show C5b-9 on neurons. c Percentage of C5b-9 and C1q positive neurons at different distances from C5b-9 or C1q positive astrocytes (mean ± S.E.M., n = 6, *P < 0.01 comparing AQP4-IgG vs. control IgG)

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