Fig. 3
From: Leucine-rich repeat kinase 2 controls protein kinase A activation state through phosphodiesterase 4
LRRK2 kinase activity affects phosphorylation of PKA RIIβ at S114. a LRRK2 WT and LRRK2G2019S KI brain lysates were subjected to immunoblotting using P-PKA RIIβ and total PKA RIIβ antibodies. Quantification of P-PKA RIIβ is normalized for total PKA RIIβ protein. Data are representative of four animals (bars represent the mean ± SEM; unpaired t test; *p < 0.05). b LRRK2 WT and LRRK2 KO brain lysates were subjected to immunoblotting using P-PKA RIIβ and total PKA RIIβ antibodies. Quantification of P-PKA RIIβ is normalized for total PKA RIIβ protein. Data are representative of three animals (bars represent the mean ± SEM; unpaired t test; *p < 0.05). c BV2 cell lysates treated with GSK, forskolin (Forsk), and DMSO as control (CTR) were subjected to immunoblotting using P-PKA RIIβ and total PKA RIIβ antibodies. Quantification of P-PKA RIIβ is normalized for total PKA RIIβ protein. Data are representative of seven independent experiments (bars represent the mean ± SEM; one-way ANOVA with Tukey’s post-test; *p < 0.05, **p < 0.01)