Fig. 3

Rotarod and open field test on Atm^+/+and Atm^−/− animals with 2-week ibuprofen administration followed by 4-day LPS injection. Atm^−/− animal traveled less distance (A) on day 1 before LPS administration. After LPS injection, both genotypes showed a significant loss of motor activity as measured by distance traveled (a) and rotation (b) on day 3 although some recovery was noted at day 5. n = 6–10 for each group. c Experimental timeline for ibuprofen experiment. d ATM deficiency significantly reduced rotarod performance of Atm^−/− animal and ibuprofen failed to improve it. e Similar results were observed in open field tests; ibuprofen failed to improve reduced motor activities in LPS injected Atm^−/− animals. n = 8–11 for each group. Two-way ANOVA was used for analyzing differences in genotype and ibuprofen treatment within an individual day. * p < 0.05, ** p < 0.01 between saline and LPS groups within the same genotype; # p < 0.05, ## p < 0.01, ### p < 0.001 between Atm^+/+ and Atm^−/− groups with the same treatment