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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: TLR4 inhibitor TAK-242 attenuates the adverse neural effects of diet-induced obesity

Fig. 4

Microglial number, morphological status, and soma size as assessed by IBA-1 immunohistochemistry in control (CTL) and high-fat diet (HFD)-fed mice treated with vehicle (Veh) or the TLR4 inhibitor, TAK-242 (TAK). ac Representative images of microglial morphology. Scale bar = 10 μm. a Resting (type 1) microglial cells are characterized by small cell bodies with numerous branching processes. Reactive microglia are either b type 2 cells with rod-shaped cell bodies and fewer, thicker projections, or c amoeboid cells with no processes or with filopodia. Densities of IBA-1 immunoreactive cells were quantified in d entorhinal cortex and in the f subiculum, h CA1, and j CA2/3 of the hippocampus. Percentages of reactive microglia (type 2 and 3 cells) were quantified in e entorhinal cortex and in the hippocampal subregions g subiculum, i CA1, and k CA2/3. l Microglial soma size was assessed specifically in CA1 of the hippocampus. Data are presented as mean (±SEM) values; n = 10/group. Vehicle-treated animals are shown in white bars, and TAK-242-treated are shown in black bars. Statistical significance is based on ANOVA followed by Bonferroni correction. *p < 0.05 relative to drug treatment-matched mice in control diet condition. #p < 0.05 relative to vehicle-treated mice in the same diet condition

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