Fig. 2
From: Protein kinase C-delta inhibition protects blood-brain barrier from sepsis-induced vascular damage
Sepsis-induced PKCδ activation and BBB barrier damage in rats are attenuated by PKCδ inhibition. a Representative Western blot images of phosphor-PKCδ membrane and cytosolic fractions in the brain samples of sham-operated, septic (CLP), or treated septic (CLP+PKCδ-i) rats. VE-cadherin was used as a marker for the membrane fraction. b Densitometry analysis of phosphorylated PKCδ (Ser643) translocation. Values are expressed as the density ratio of the membrane to the cytosolic fraction. c PKCδ inhibition (PKCδ-i) also attenuates sepsis (CLP) induced Evans blue (EB) dye extravasation in rat brain. Data are presented as mean ± SEM (n = 3). *p < 0.05 compared to sham and CLP+PKCδ-i by ANOVA with Tukey-Kramer post hoc