Fig. 8

Analysis of lncRNA AK006025 interaction with CBP/P300 to regulate CXCL9/10/11 cluster gene expression in astrocytes treated with Nef. a RIP assay analysis of the interaction of AK006025 with NF-κB p65 and CBP/P300 in astrocytes treatment with Nef protein for 3 h. NC: natural control. ***P < 0.001, vs NC. These data are from three independent experiments. b ChIP assay analysis of NF-κB p65, CBP/P300, RNA Pol II, and H3K27ac enrichment on the promoter of CXCL9/10/11 gene cluster in astrocytes treatment with Nef protein for 3 h. **P < 0.01, ***P < 0.001, vs NC. Error bars represent means ± SEM. These data are from three independent experiments. c ChIP assay analysis of NF-κB p65, CBP/P300, and H3K27ac enrichment on the promoter of CXCL9/10/11 gene cluster in astrocytes transfected siRNAs for 48 h, followed by treatment with Nef protein for 3 h. si-AK: si-AK006025. *P < 0.05, **P < 0.01, ***P < 0.001, vs his-Ctrl. Error bars represent means ± SEM. These data are from three independent experiments