Fig. 7
From: Peripheral myeloid cells contribute to brain injury in male neonatal mice
Neuroprotection in males only following myeloid cell depletion. Brain tissue was immuno-labelled for neuronal and white matter tissue using antibodies against MAP2 and MBP, respectively, and tissue loss was measured 14 days after hypoxia-ischemia in mice treated with saline (male n = 17; female n = 19) or RB6-8C5 antibody (male n = 14; female n = 20). a Representative images of MAP2-stained sections of the brain at three levels (L1–L3) obtained from male mice treated with saline or RB6-8C5 after HI. b, c RB6-8C5 antibody treatment reduced the MAP2+ neuronal tissue loss in male (b) and but not in female (c) mice. d, e Representative images of MBP-stained sections of the brain at three levels (L1–L3) obtained from male mice treated with saline or RB6-8C5 after HI. e, f RB6-8C5 antibody treatment did not significantly affect the MBP+ myelin loss in male (e) and female (f) mice. Values are presented as the mean ± SD. Brain injury comparisons were performed using multiple unpaired Student’s t tests at each brain level; p values were corrected for multiple comparison using the Holm-Sidak method. *p ≤ 0.05