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Table 1 Antibodies for immunohistochemistry and flow cytometry

From: Peripheral myeloid cells contribute to brain injury in male neonatal mice

Application

Antigen

Host

Clone/target

Reactivity*

Conjugate

Company

Product number

Dilution**

Immunohistochemistry

GFP

Rabbit

Poly

Mouse

A488

Invitrogen

A21311

1:200

CD31

Goat

Poly

Mouse

–

R&D systems

AF3628

1:200

Iba1

Goat

Poly

Mouse

–

Abcam

Ab5076

1:500

Ly6G

Rat

Mono (1A8)

Mouse

–

BioLegend

127602

1:250

IgG

Donkey

Poly

Goat

CF555

VWR

89138-464

1:1000

IgG

Goat

Poly

Rat

CF555

Sigma

SAB46000070

1:1000

Flow cytometry

CD11b

Rat

M1/70

Mouse

PE-Cy7

BioLegend

101216

0.25 μg

CD45

Rat

30-F11

Mouse

APC-CY7

BD

557659

0.25 μg

GR-1

Rat

RB6-8C5

Mouse

PerCp-Cy5.5

eBiosciences

45-5931

0.25 μg

Ly6C

Rat

HK1.4

Mouse

APC

eBiosciences

17-5932

0.25 μg

CD16/CD32

Rat

2.4G2

Mouse

–

BD

553142

0.1 μg

  1. *Where antibody is reactive to multiple species, only relevant species are listed
  2. **For flow cytometry, given values represent microgram antibody per 106 cells