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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Omega-3 polyunsaturated fatty acid attenuates traumatic brain injury-induced neuronal apoptosis by inducing autophagy through the upregulation of SIRT1-mediated deacetylation of Beclin-1

Fig. 3

(a,b) Immunofluorescence staining showed that LC-positive neurons significantly increased following ω-3 PUFA supplementation. While, the autophagic inhibitor, 3-MA, attenuated LC-positive neuron expressions. Representative photomicrographs of LC-positive neurons are shown (× 400). (c,d) Western blot analysis showed that expression levels of autophagic markers (LC3-II, Beclin-1, ATG-3, and ATG-7) were dramatically increased in the TBI+ω-3 PUFA group 7 day after TBI (p < 0.05), while 3-MA inhibited ω-3 PUFA-induced autophagy responses. Values are expressed as mean ± standard deviation (n = 6 per group). N.S., p > 0.05, *p < 0.05, **p < 0.01. Scale bars = 50 μm

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Journal of Neuroinflammation

ISSN: 1742-2094