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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Reduced AMPK activation and increased HCAR activation drive anti-inflammatory response and neuroprotection in glaucoma

Fig. 1

Iba1 expression in the retina and ON of D2 and D2G mice. a Immunohistochemical analysis of Iba1 (red) expression in untreated (Unt) and keto D2 and D2G retinas and ONs. DAPI (blue) stains nuclei. b, c Percentage of mean fluorescence of Iba1 in the inner retina (b), *p = 0.0369, ***p = 0.0001, and in the region of interest (ROI) of the proximal ON (c), *p = 0.0415, ***p = 0.0001. d, e Quantification of Iba1+ microglia in the inner retina (d), *p = 0.0341, ***p = 0.0001, and in the ROI of the proximal ON (e), *p = 0.0245, ***p = 0.0001. For b–e, five retinas and ON from each group were analyzed, with five sections from each individual retina or ON. f Western blot analysis of Iba1 protein expression in retinas. g Bar graph with densitometry of Iba1 levels normalized to actin (β isoform) levels, *p = 0.0293, **p = 0.0022; n = 3 blots per group, each with independent samples. h–j Capillary tube electrophoresis of Iba1 protein in ON, normalized to actin levels. Typical capillary electrophoresis output, showing digital view of bands within capillary tube (h), digital graphical plot of HRP signal produced by HRP-conjugated secondary antibody that is bound to the antigen of interest (i); bar graph represents the areas of HRP signal normalized to areas of actin HRP signal (j), *p = 0.0310, ***p = 0.0013; n = 3 biological replicates per group. All bar graphs are the mean ± SEM, n = 5, analyzed by two-way, unpaired t test. Scale bar, 20 μm

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