Fig. 6

Analysis of NLRP3, IL-1β, and ARRB2 expression in untreated (Unt) and Keto D2 and D2G retina and ON. a, b Nlrp3 (a), *p = 0.0219, and Il-1β (b), *p = 0.0151 mRNA levels normalized to Hprt mRNA levels determined by qRT-PCR; n = 8 samples per group. c Western blot analysis of NLRP3 and IL-1β protein levels in the retina. d, e Quantification by densitometry of NLRP3 (d), ***p = 0.0001, ***p = 0.0001, and mature IL-1β (e), ***p = 0.0001, ***p = 0.0001 protein levels normalized to β-actin levels; n = 3 blots per group, each with independent samples. f IL-1β protein levels in the ON analyzed by ELISA, **p = 0.0092; n = 8 samples per group. g Western blot analysis of ARRB2 protein levels in the retina. h Quantification by densitometry of ARRB2 protein normalized to β-actin; n = 3 blots per group, each with independent samples. i Capillary tube electrophoresis of ARRB2 protein levels in the optic nerve normalized to β-actin ***p = 0.0001, ***p = 0.0001; n = 3 ON per group. All bar graphs are presented as the mean ± SEM, n = 5–9, analyzed by two-tailed unpaired t test