Skip to main content
Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Glial-neuronal signaling mechanisms underlying the neuroinflammatory effects of manganese

Fig. 6

Loss of IKK2 expression in astrocytes reduces apoptosis in N2A cells following treatment with conditioned medium from mixed glial cultures. a GCM from mixed glial cultures containing WT astrocytes (black bars) treated with 100 μM MnCl2 decreases N2A viability compared to control (~ 25%), whereas neuronal viability is largely preserved (indicated by lack of statistically significant loss) in N2A cells treated with GCM from mixed glial cultures containing IKK2 KO astrocytes (white bars) compared to control. b, c Mn-GCM (WT, black bars) causes an increase in Annexin V+ and PI+ N2A cells, which is reduced in N2A cells treated with GCM from mixed glial cultures containing IKK2 KO astrocytes (white bars). Two-way ANOVA analyses performed. Data depicted as ± S.E.M. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 (n ≥ 4 per treatment group; across ≥ 3 independent experiments)

Back to article page