Fig. 3

Cytoplasmic mislocalization of TDP-43 in neuronal and glial cells causes upregulation of inflammatory response 72 h after MCAO in aging. a Representative photographs 48 h after MCAO after real-time imaging of TLR2 induction. The color calibrations at the right are photon counts. b Data plotted was obtained measuring the photon emission. Red (3 month old) and green (12 month old) lines indicate TLR2 induction at different time points post MCAO. c Western blot of cortical lysates from 3- and 12-month-old mice using Iba1 in control and 72 h after MCAO reveals an increase in the levels of Iba1 in 12-month-old mice. Actin is used as loading control. d Western blot of nuclear lysates from 3- and 12-month-old wild-type mice using phospho-P65 in control and 72 h after MCAO reveals an increase in the levels of phospho-P65 in 12-month-old mice. P84 is used as loading control. e Levels of inflammatory cytokines like IL-1β, IL-4, IL-6, IL-10, IL-17, TNF-α, CCL5, and GM-CSF were significantly increased in 12-month-old mice 72 h after MCAO. Quantified data in the figure was presented as mean ± SEM and statistical significance between the groups was achieved using one-way ANOVA followed by Tukey’s multiple comparison test and depicted as ***p < 0.001, *p < 0.05