Fig. 5

Overexpression of TDP-43 increases brain damage and inflammatory response after stroke. a Representative image of cresyl violet staining showing stroke area in 3-month-old WT and TDP-43^A315T 72 h after MCAO. b Quantification of stroke area reveals an increase in the ischemic lesion in TDP-43^A315T compared to WT mice. c Western blots of cytoplasmic lysates coming from 3- and 12-month-old WT and TDP-43^A315T mice using cleaved caspase-3. d Quantification of immunoblots 72 h after MCAO reveal a significant increase in the levels of cleaved caspase-3 in 12-month-old TDP-43^A315T mice when compared to the 3-month TDP-43^A315T or the 12-month-old WT control. e Double immunofluorescence of the brain cortex sections of 3- and 12-month-old TDP-43^A315T mice 72 h after MCAO using caspase-3 antibody (green) and NeuN (red) antibody show neuronal apoptosis in both age mice. f Expression of P65 by revealed by Western blot in control mice (3- and 12-month-old WT and TDP-43^A315T). g Expression of P65 by revealed by Western blot 72 h after MCAO (3- and 12-month-old WT and TDP-43^A315T). Normalized densitometry values of Western blot reveal a general significant increase in the levels of P65 after MCAO in either age group or mice. 3- and 12-month-old TDP-43^A315T show a higher P65 base level compared to the 3-month-old WT control. Quantified data in the figure was presented as mean ± SEM and statistical significance between the groups was achieved using unpaired t test and depicted as ***p < 0.001, **p < 0.01