Fig. 2

KCa3.1 upregulation in Aβ-induced RA and the brains of AD patients. a Primary astrocytes were stimulated with 5 μM Aβ and lysates were subjected to Western blot analysis with antibodies against KCa3.1, Orai1, and STIM1. β-actin was used to confirm equal loading. b Data are presented as the mean ± SEM (n = 5). The OD values of KCa3.1, Orai1, and STIM1 were normalized to that of β-actin. *p < 0.05 vs. control cells (unpaired, two-tailed Student’s t test). c Double immunofluorescence staining of KCa3.1 with Orai1 in astrocytes with or without stimulation of 5 μM Aβ. Nuclei were stained in blue with DAPI. Scale bar: 25 μm. d Brain lysates from control and AD samples were subjected to SDS-PAGE and immunoblotted with antibodies against KCa3.1, Orai1, and STIM1. e Data are presented as the mean ± SEM (n = 5). The OD values of KCa3.1, Orai1, and STIM1 were normalized to that of β-actin. *p < 0.05 vs. controls (one-way ANOVA followed by the Dunnett’s multiple comparison test). f Double immunofluorescence staining of KCa3.1 with Orai1 in the brain sections of control and AD patients. Arrows indicate colabeling of KCa3.1 with Orai1. Nuclei were stained blue with DAPI. g, h Double immunofluorescence staining of KCa3.1 with GFAP in the brain sections of control and AD patients (g) or WT and APP/PS1 mice (h). Scale bar: 50 μm