Fig. 8
Pannexin-1 is not involved in permeabilization of human microglia. a The uptake of YO-PRO-1 into human microglia is independent of pannexin-1 channels. Microglia were pre-incubated for 30 min with the pannexin-1 inhibitors carbenoxolone (CBX, 20 μM), probenecid (PB, 5 mM), or 10panx (300 μM) and subsequently assessed for YO-PRO-1 uptake after 15 min in the presence of BzATP (300 μM). None of the antagonists had a significant effect on dye uptake (n = 3 separate experiments). b Representative patch-clamp current tracing of microglia stimulated with ATP (10 mM, 3 s) before and after 2-min incubation with CBX (20 μM). c Current amplitude (as fraction of control #1: the current amplitude before antagonist application) induced by ATP (5 mM) after 2-min incubation with CBX (20 or 100 μM) was not significantly different from control #2. Control #2 represents the IATP measured after 2-min incubation in control ECS solution