Fig. 1

Susceptibility and replication kinetics in differentiated hNSC neuron/astrocyte co-cultures. hNSCs were primed and differentiated into mature neuron/astrocyte co-cultures for 9 days prior to infection with mock, 0.1, 1, or 10 MOI of LACV. a Co-cultures were imaged at various times post-infection via phase contrast microscopy. b Supernatant was collected at various times post-infection and titrated via plaque assay. c Cells were lysed and RNA was collected and qRT-PCR was used to determine viral replication. Anti-LACV N gene primers were used, and levels were determined as fold change relative to the mock-infected background