Fig. 2

Identification of LACV target cells in neuron/astrocyte co-cultures. Neuron/astrocyte co-cultures were infected with 0.1, 1, and 10 MOI of LACV and formalin fixed at various times post-infection. a Cells were then stained for DAPI, LACV Gc protein, and either neuronal marker MAP2 or astrocytic marker GFAP. Representative images from the mock-infected and 1 MOI LACV-infected group are shown. b The percentage of neurons and astrocytes infected was determined over the course of infection for the 0.1 MOI group. The 1 and 10 MOI are available in Additional file 2: Figure S2a. c The percentage of infected cells expressing either MAP2 or GFAP was quantified for the set of cells infected with 0.1 MOI. The 1 and 10 MOI have similar trends and are available in Additional file 2: Figure S2b. *P < 0.05, **P < 0.01, ****P < 0.0001