Fig. 5

The expression levels of PDE-5 were studied using Western blot analysis. Astrocytes were cultivated in 5.5 mM glucose the whole cultivation period. The astrocytes were incubated with LPS (10 ng/ml) for 24 h or incubated with LPS for 24 h followed by LPS and sildenafil (Sild) (1 μM) for another 24 h. n = 3. Representative image of Western blot membrane is presented