Fig. 6

Effects of KIT 17 on mRNA expression and protein synthesis of mPGES-1 (a, c) and COX-2 (b, d) in LPS-stimulated primary microglial cells. a, b Cells were pre-treated with different concentrations of KIT 17 for 30 min before stimulating with LPS. After 4 h, mPGES-1 (a) and COX-2 (b) were measured by qPCR. Western blot analysis of protein levels of mPGES-1 (c) or COX-2 (d) in cells pre-treated with different concentrations of KIT 17 for 30 min following LPS stimulation for 24 h. Protein levels were normalized to β-actin loading control (n = 3–4). *p < 0.05, **p < 0.01, and ***p < 0.001 with respect to LPS (one-way ANOVA followed by the Newman-Keuls post hoc test)