Fig. 7
From: Anti-neuroinflammatory effects of GPR55 antagonists in LPS-activated primary microglial cells
Effects of KIT 17 on COX-1 (A) and COX-2 (b) enzyme activity in primary microglia cells. a For COX-1 activity assay, cells were treated with different concentrations of KIT 17 for 15 min before addition of 15 μM of arachidonic acid. PGE2 in the supernatants was measured after additional 15 min. b Cells were stimulated for 24 h with LPS (10 ng/mL) and then treated with different concentrations of KIT 17 for 15 min. Then, 15 μM of arachidonic acid was added and PGE2 in the supernatants was measured by enzyme immune assay (EIA). Data are expressed as mean ± S.E.M. of at least 3 independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001 with respect to control (one-way ANOVA followed by the Newman-Keuls post hoc test)