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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Anti-inflammatory and anti-oxidant mechanisms of an MMP-8 inhibitor in lipoteichoic acid-stimulated rat primary astrocytes: involvement of NF-κB, Nrf2, and PPAR-γ signaling pathways

Fig. 2

M8I suppressed the expression of pro-inflammatory molecules in LTA-stimulated astrocytes. a Cells were treated with LTA (10 μg/ml) for 1 or 3 h. MMP-8 protein expression was detected by Western blot in conditioned media (CM) and cell lysates. Representative blots (upper panel) and quantification data (lower panel) are shown. b MMP-8 activity assay data. Primary astrocytes were treated with M8I for 1 h before stimulation with LTA (10 μg/ml). One hour later, the supernatants were collected to measure MMP-8 activity using the SensoLyte® 520 MMP-8 assay system. c The effect of M8I on TNF-α, IL-6, and NO production in cells treated with LTA for 24 h. d The effect of M8I on the mRNA expression of iNOS, cytokines, and TLR2 was determined by RT-PCR analysis. The data are the mean ± S.E.M. of three independent experiments. #P < 0.05, vs. control samples. *P < 0.05, vs. LTA-treated samples

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