Fig. 6

M8I increased the DNA binding and transcriptional activities of Nrf2. a, b EMSA for Nrf2 DNA binding activity. Nuclear extracts were prepared from astrocyte cells treated with M8I in the presence or absence of LTA (10 μg/ml) for 6 h and incubated with the probe (ARE). The arrow indicates a DNA–protein complex of Nrf2. c Effect of M8I on ARE-luc reporter gene activity. Astrocytes were transfected with the reporter plasmid (ARE-luc) and treated with M8I in the presence or absence of LTA for 6 h, and luciferase assay was performed. The data are the means ± S.E.M. for three independent experiments. ^#P < 0.05, vs. control samples. ^*P < 0.05, vs. LTA-treated samples