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Table 1 List of primers used in quantitative RT-PCR analysis, antibodies and lectins used in the experiments and their working dilutions

From: Persistent reduction in sialylation of cerebral glycoproteins following postnatal inflammatory exposure

Gene

Forward (5′ to 3′)

Reverse (5′ to 3′)

Reference

 Gapdh

AAGGTCGGTGTGAACGGATT

TGAACTTGCCGTGGGTAGAG

[63]

 Neu1

GTAGCACGTGGTCCTCTACG

ATCGTGATCGTGTTTGGGCT

Primer Blast

 Neu3

CTCAGTCAGAGATGAGGATGCT

GTGAGACATAGTAGGCATAGGC

[64]

 Neu4

CCGGACTGTGGTTGGTAGAC

ACCAGGGAGCGTAAAGCAAT

Primer Blast

Antibody/lectin

Working dilution

Catalog number, company

 Anti GFAP (GA5), mouse mAb

1:300

3670, Cell Signaling Technology

 Anti-PSA, mouse mAb

1:600

[65]

 Anti-NeuN, mouse mAb

1:200

MAB377, EMD Millipore Corporation

 Anti-NeuN, guinea pig polyclonal

1:300

ABN90P. EMD Millipore Corporation

 Anti-LAMP1 antibody—lysosome marker

1:1500

ab24170, Abcam

 Goat anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor 488

1:1500

A-11034, Thermo Fisher Scientific

 Goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody, Alexa Fluor 555

1:1500

A-21422, Thermo Fisher Scientific

 Goat anti-mouse IgG (H+L), cross-adsorbed secondary antibody, Alexa Fluor 488

1:500

A11001, Invitrogen

 Donkey anti-guinea pig IgG antibody, Cy3 conjugate 555

1:750

AP193C, EMD Millipore Corporation

 Isolectin GS-IB4 from Griffonia simplicifolia, Alexa Fluor® 568 conjugate

1:200

I21412, Thermo Fisher Scientific

 Biotinylated peanut agglutinin lectin

1:10,000

B-1075, Vector Laboratories

 Biotinylated Maackia amurensis lectin II

1:20,000

B-1265, Vector Laboratories

 Streptavidin-horseradish peroxidase conjugate

1:1000

RPN1231-100UL, GE Healthcare Life Sciences