|
Gene
|
Forward (5′ to 3′)
|
Reverse (5′ to 3′)
|
Reference
|
|
Gapdh
|
AAGGTCGGTGTGAACGGATT
|
TGAACTTGCCGTGGGTAGAG
|
[63]
|
|
Neu1
|
GTAGCACGTGGTCCTCTACG
|
ATCGTGATCGTGTTTGGGCT
|
Primer Blast
|
|
Neu3
|
CTCAGTCAGAGATGAGGATGCT
|
GTGAGACATAGTAGGCATAGGC
|
[64]
|
|
Neu4
|
CCGGACTGTGGTTGGTAGAC
|
ACCAGGGAGCGTAAAGCAAT
|
Primer Blast
|
|
Antibody/lectin
|
Working dilution
|
Catalog number, company
|
|
Anti GFAP (GA5), mouse mAb
|
1:300
|
3670, Cell Signaling Technology
|
|
Anti-PSA, mouse mAb
|
1:600
|
[65]
|
|
Anti-NeuN, mouse mAb
|
1:200
|
MAB377, EMD Millipore Corporation
|
|
Anti-NeuN, guinea pig polyclonal
|
1:300
|
ABN90P. EMD Millipore Corporation
|
|
Anti-LAMP1 antibody—lysosome marker
|
1:1500
|
ab24170, Abcam
|
|
Goat anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor 488
|
1:1500
|
A-11034, Thermo Fisher Scientific
|
|
Goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody, Alexa Fluor 555
|
1:1500
|
A-21422, Thermo Fisher Scientific
|
|
Goat anti-mouse IgG (H+L), cross-adsorbed secondary antibody, Alexa Fluor 488
|
1:500
|
A11001, Invitrogen
|
|
Donkey anti-guinea pig IgG antibody, Cy3 conjugate 555
|
1:750
|
AP193C, EMD Millipore Corporation
|
|
Isolectin GS-IB4 from Griffonia simplicifolia, Alexa Fluor® 568 conjugate
|
1:200
|
I21412, Thermo Fisher Scientific
|
|
Biotinylated peanut agglutinin lectin
|
1:10,000
|
B-1075, Vector Laboratories
|
|
Biotinylated Maackia amurensis lectin II
|
1:20,000
|
B-1265, Vector Laboratories
|
|
Streptavidin-horseradish peroxidase conjugate
|
1:1000
|
RPN1231-100UL, GE Healthcare Life Sciences
|
