Fig. 1

Impaired hippocampus-dependent long-term memory in J20 and J20xLcn2 KO mice, no differences in working memory. a Alternation (%) in the Y-maze spontaneous alternation test. b Number of arm entries in the Y-maze spontaneous alternation test (10 min). Tested with one-way ANOVA with Tukey’s multiple comparisons test. n = 13–17 mice per group (WT n = 17, J20 n = 13, Lcn2 KO n = 16, J20xLcn2 KO n = 15). c Discrimination index (%) in the novel location recognition (NLR) training and d in the NLR test, that was performed 24 h after the NLR training. Dashed line indicates 50% chance level. Of note, one Lcn2 KO and one J20xLcn2 KO mouse had to be excluded from the analysis due to lack of exploratory behavior. n = 13–17 mice per group (WT n = 17, J20 n = 13, Lcn2 KO n = 15, J20xLcn2 KO n = 14). Tested with one-way ANOVA with Tukey’s multiple comparisons test. e Escape latency (s) and (f) swim distance (cm) in the training phase of the Morris water maze (MWM). n = 13–17 mice per group (WT n = 17, J20 n = 13, Lcn2 KO n = 16, J20xLcn2 KO n = 15). Tested with two-way repeated measures ANOVA. g Number of platform crossings and h time spent in the pool quadrants during the probe trial (24 h after the last training trial). n = 13–17 mice per group (WT n = 17, J20 n = 13, Lcn2 KO n = 16, J20xLcn2 KO n = 15). Tested with one-way ANOVA with Tukey’s multiple comparisons test. *p < 0.05; **p < 0.01; ***p < 0.0001 compared to WT. No significant differences were present between J20 and J20xLcn2 KO mice