Fig. 3

Clear plaque formation and activation of astrocytes and microglia in J20 and J20xLcn2 KO mice. a Representative examples of immunohistochemical staining for Aβ with 6e10 antibody (× 50 magnification, scale bar: 500 μm), and (in the outer right column) quantification of the coverage of total hippocampal Aβ staining. b Representative examples of immunohistochemical staining for GFAP (× 100 magnification, scale bar: 300 μm), and (in the outer right column) quantification of the coverage of GFAP staining in the dentate gyrus and CA1 region of the hippocampus (combined). c Representative examples of immunohistochemical staining for Iba1 (× 200 magnification, scale bar: 150 μm), and (in the outer right column) quantification of microglial activity in the dentate gyrus of the hippocampus. n = 8–10 mice per group (WT n = 10, J20 n = 8, Lcn2 KO n = 9, J20xLcn2 KO n = 9), 3–6 hippocampi were analyzed per mouse. Tested with one-way ANOVA with Tukey’s multiple comparisons test. **p < 0.01 and ***p < 0.0001 compared to WT. No significant differences were present between J20 and J20xLcn2 KO mice