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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Lipocalin 2 contributes to brain iron dysregulation but does not affect cognition, plaque load, and glial activation in the J20 Alzheimer mouse model

Fig. 4

Increased hippocampal iron accumulation in J20 mice, and less severe iron accumulation in J20xLcn2 KO mice. a Representative examples of histochemical staining for iron by enhanced Perls’ reaction. Images were acquired and analysis was performed at × 100 magnification (scale bar: 300 μm). Detailed images were acquired at × 400 magnification (scale bar: 50 μm). Examples include a focus on the CA1 and the dentate gyrus regions of the hippocampus. b Quantification of the coverage of iron-positive staining, in the stratum oriens and pyramidal layer of the CA1 region, and in the inner blade and granular layer of the dentate gyrus. Of note, due to regional differences in intensity of the iron staining, the iron staining in the CA1 pyramidal and DG granular cell layers was analyzed with a different detection threshold than the detection threshold used to analyze the other hippocampal sub-regions. This explains the higher relative coverages of iron staining found for the CA1 pyramidal and DG granular cell layers, as compared to other hippocampal sub-regions. c Quantification of the optical density of iron-positive staining, in the stratum oriens and pyramidal layer of the CA1 region, and in the inner blade and granular layer of the dentate gyrus. n = 8–9 mice per group (WT n = 9, J20 n = 8, Lcn2 KO n = 9, J20xLcn2 KO n = 9), 3–4 hippocampi were analyzed per mouse. Tested with Kruskal-Wallis test with Dunn’s multiple comparisons post-hoc test. *p < 0.05 and **p < 0.01

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