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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Colony-stimulating factor 1 receptor inhibition prevents disruption of the blood-retina barrier during chronic inflammation

Fig. 2

Accumulation of microglia/macrophages around retinal vessels in LPS-challenged mice. Experimental setup (a) and representative autofluorescence images of microglia/macrophages in the retina at baseline (b), after the fourth LPS injection (c) and 3 days after the last LPS injection (d). GFP-positive cells were identified as white signal on the autofluorescence images. One representative retina out of ≥ 8 is presented for each time point. Retinal whole mounts from untreated (e, control) and LPS-treated Cx3cr1gfp/+ mice stained with GFP at day 4 (f) and at day 7 (g) after the first LPS injection. One representative retina out of ≥ 4 is presented for each time point. Higher magnification of the delineated areas in e, f, and g are depicted in h, i, and j, respectively. The numbers of GFP-positive cells were elevated in the retina at day 4 of the LPS challenge and 3 days after the last LPS challenge, compared to the baseline (k; **p < 0.01, ***p < 0.0001; n = 13 eyes for each time point; repeated measures one-way ANOVA followed by Tukey’s post hoc analysis). The area occupied by microglia cells in the retina was elevated after the LPS challenge and was decreased 3 days after the termination of the inflammatory stimulus (l; n = 14 eyes for each time point; *p < 0.05, **p < 0.01, ***p < 0.001; repeated measures one-way ANOVA followed by Tukey’s post hoc analysis). Arrows represent the location of retinal vessels. Scale bars: 500 μm (e, f, g); 200 μm (h, i, j)

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