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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Colony-stimulating factor 1 receptor inhibition prevents disruption of the blood-retina barrier during chronic inflammation

Fig. 3

Activation and migration of retinal microglia/macrophages after LPS challenge in Cx3cr1gfp/+ mice. In control retinas (a), Iba-1-positive cells were mainly found in the inner and outer plexiform layer (IPL and OPL, respectively). In LPS-challenged retinas (b, c), Iba-1-positive microglia/macrophages were also detected in the proximity of the sub-retinal fluid accumulation (red arrows in b) and in the distal ONL (yellow arrows in b and c). Iba-1-positive cells migrating towards the outer retina were also detected (red arrow in c). In control retinal whole mounts, GFP-positive microglia cells have a ramified morphology typical for resting microglia (d). After LPS challenge, activated GFP-positive cells with retracted processes and round cytosol were detected in the retina (e). One representative retina out of ≥ 4 is presented for each time point. Scale bars: 100 μm. GCL, ganglion cell layer; IPL, inner plexiform layer; OPL, outer plexiform layer; RPE, retinal pigment epithelium

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