Fig. 7

Er-NPCs engraftment counteracts macrophages invasion and allows the switch from M1 to M2 subtype. Immunohistochemistry analysis of coronal sections obtained from the brain of healthy control animals, treated with MPTP and with MPTP + Er-NPCs ipsilateral and contralateral to the transplant site and with MPTP + Er-NPCs + aEPO ipsilateral to the transplant site. The brain sections were labeled with the antibody CD68 (a), CD86 (b), and CD206 (c) in red. Scale bars represent 50 μm (CD68, CD86, and CD 206). Quantification was done by ImageJ picture analysis software and reports the fluorescence intensity/pixel expressed by the studied markers (n = 6 mice each group; three fields for mouse) (see the “Methods” section). ***p < 0.001; **p < 0.01 vs CTRL; ^###p < 0.001; ^##p < 0.01; ^#p < 0.05 vs MPTP; °°°p < 0.001; °°p < 0.01; °p < 0.05 vs MPTP + Er-NPCs ipsilateral; ^§§§p < 0.001; ^§§p < 0.01 vs MPTP + Er-NPCs contralateral (d)