Fig. 3

βCaMKII is upregulated in CA1 regions in a rat model of depression. a Western blot analysis showing protein levels of CaMKII in CA1 of rats. b The phosphorylation levels of CaMKII in CA1 of rats. c The phosphorylation levels of βCaMKII in CA1 of rats. d Immunofluorescence analysis of βCaMKII in CA1 of two stress paradigms. Nuclei (blue) are stained with DAPI. Scale bar is 50 μm. e Histograms showing the fluorescence intensities of βCaMKII in CA1 of each group. f QPCR analysis of βCaMKII mRNA in CA1 of two stress paradigms. N = 6 per group. Data are presented as the means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 CUMS (or LPS) vs control group; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 CUMS (or LPS)+Cele vs depression group; ^&P < 0.05, ^&&P < 0.01, ^&&&P < 0.001 CUMS (or LPS)+FLX vs depression group (Cele, celecoxib; FLX, fluoxetine)