Fig. 7

Unaltered iba-1 immunostaining in the contralateral VPM (cVPM) and MD and intralaminar thalamic nuclei (IL) 14 days after spinal nerve ligation. Microphotographs in A1 and A2 illustrate the delineation of the ventral posteromedian thalamic nucleus (VPM; outlined in white in A1) and the mediodorsal thalamic nucleus (MD; outlined in purple in A2) and the group of the intralaminar thalamic nuclei (outlined in blue in A2) that includes the central medial thalamic nucleus (CM), the paracentral thalamic nucleus (PC), and the central lateral thalamic nucleus (CL) on a rat brain section treated for visualizing acetylcholinesterase activity. The outlines were then applied on an adjacent section treated for the immunohistofluorescent detection of the iba1. Microphotographs in B are examples of iba-1 immunostaining (green) in the cVPM of naïve, sham, and SNL animals 14 days after the beginning of the experiments. Morphometric analysis (SNL and sham: n = 6; naïve: n = 4; 3 slices per animal) reveals that the treatment does not alter the iba-1 immunofluorescent surfaces in the cVPM (C: one-way ANOVA F(2, 13) = 4.244, p = 0.0381 but no significant differences with the Tukey’s multiple comparison test), MD (D: one-way ANOVA F(2, 13) = 0.07, p = 0.9322), and IL (E: one-way ANOVA F(2, 13) = 0.6494, p = 0.5385)