Table 1 Quantitative real time PCR primers
Gene name | Primer name | GenBank ID | Forward primer sequence | Reverse primer sequence |
|---|---|---|---|---|
Glial fibrillary acidic protein | Gfap | NM_017009 | GGTGTGGAGTGCCTTCGTATTAG | GGGACACTTTCAGCTCCATTTCT |
S100 calcium binding protein, beta polypeptide | S100b | NM_013191 | GAGGAATGAAGGGCCACTGA | CCCTAGGCACCAGCAGGTC |
Glutamine synthetase | GS | NM_017073 | ATTCCACGAAACCTCCAACATC | TCTCCTGGCCGACAATCC |
Cluster of differentiation 11b/integrin alpha M | CD11b/Itgam | NM_012711 | TGAAGGCTCAGACAGAGACCAAA | GCTGCCCACAATGAGTGGTAC |
Ionized binding adaptor molecule 1 | Iba-1 | NM_017196 | CAACAAGCACTTCCTCGATGATC | TGAAGGCCTCCAGTTTGGACT |
Toll-like receptor 4 | Tlr4 | NM_019178 | AGCAATGTGATGAAACCCCATAC | GATTCTTTGCCTGAGTTGCTTAATT |
Cathepsin S | Ctss | NM_017320 | GTGGTTGGCTATGGGACTCTTG | CCAAAGTGAAGGCCCCAACT |
Chemokine (C-X3-C motif) ligand 1/fractalkine | Cx3cl1 | NM_134455 | CAGCCAGTGACTCACTCCTTGA | CTGGCTTCCTCACTCTGGGA |
Chemokine (C-X3-C motif) receptor 1/fractalkine receptor | Cx3cr1 | NM_133534 | GAACCGGAAGAAGGCCAGAG | GCGTCCAGAAGAGGAAGAAGAC |
β actin | Actb | NM_031144 | CGGCAATGAGCGGTTCC | TGCCACAGGATTCCATACCC |
Peptidylprolylisomerase A | Ppia | NM_017101 | ACCCCATCTGCTCGCAATAC | GGAATGAGGAAAATATGGAACCC |
Succinate dehydrogenase complex, subunit A | Sdha | NM_130428 | TGCGGAAGCACGGAAGGAGT | CTTCTGCTGGCCCTCGATGG |
Valosin-containing protein | Vcp | NM_053864 | GAGGTTTTGGCAGCTTCAGATT | ACCTCCACTGCCCTGACTTG |