Fig. 10

Effect of higher-dose minocycline treatment on CD68-immunolabeled cells at 3 days after photothrombotic stroke. a Representative image of CD68 (red) and NeuN (green) double-immunolabeled coronal section at the infarct boundary of a vehicle-treated rat. The infarct is visible in the upper part of the image. The scale bar = 200 μm. b Effects of the higher-dose minocycline treatment on CD68-positive cells within the peri-infarct tissue at 3 days following photothrombotic stroke (n = 6 to 7 per group). The CD68-positive particle count was assessed within 0.3 mm of the infarct (Peri-infarct) and between 0.3 and 0.6 mm from the infarct (Peri-infarct + 0.3 mm). Two-way analysis of variance revealed a highly significant effect of both treatment (p < 0.01) and tissue location (p < 0.001) on CD68-positive particle count at 3 days after stroke. There was also a strong interaction between the two factors (p < 0.001) reflecting the larger decrease in particle density induced by minocycline in the tissue immediately adjacent to the infarct compared with the other regions examined