Fig. 5

Changes in the pattern of Iba1-immunolabeling after photothrombotic stroke. a Regional differences in area fraction at 24 h. b–d Changes in b area fraction, c Iba1-positive particle count, and d Iba1-positive particle size in peri-infarct tissue, tissue 1 mm from the infarct and corresponding contralateral tissue between 3 h and 7 days. For all panels, n = 4 per data point; *p < 0.05, **p < 0.01, ***p < 0.001 compared with Contra01 (one-way ANOVA with Tukey’s HSD post-hoc test). At 24 h, there was also a significant difference between each of the peri-infarct regions and tissue located 1 mm from the infarct (at ipsi01a + 1 mm) for area fraction (Ipsi01a: p < 0.05; Ipsi01b: p < 0.01) and particle count (p < 0.01 for each of the peri-infarct regions). The ROIs that were analyzed are identified in Fig. 2a