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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Peripheral adaptive immunity of the triple transgenic mouse model of Alzheimer’s disease

Fig. 2

Representative flow cytometry plots for bone marrow hematopoietic stem cells. Long-term reconstituting (LTR, lin/Sca-1+/cKit+/CD34) and short-term reconstituting (STR, lin/Sca-1+/cKit+/CD34+) hematopoietic stem cells were quantified in bone marrow. The debris and dead cells were excluded before the selection of lineage negative cells. The Sca-1+/cKit+ double-positive cells were then gated. Finally, LTR and STR were separated according to their expression of the CD34 marker. Representative results from (upper panels) non-transgenic (NTg) and (center panels) 3xTg-AD mice are presented. (Lower panels) The fluorescence minus one (FMO) controls were used for appropriate gating of the cell populations

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