Fig. 3
From: Peripheral adaptive immunity of the triple transgenic mouse model of Alzheimer’s disease
Increased activation of B lymphocytes in the 3xTg-AD mouse. a (Upper panels) The rise of serum and cortex immunoglobulin G (IgG) concentrations (ELISA), and (lower panels) percentage of IgG-secreting cells (ELISPOT, spleen) and plasma cells (flow cytometry, bone marrow) strongly suggest elevated activation of B lymphocyte in the 3xTg-AD model compared to non-transgenic animals (NTg, N = 8–12 12-month-old mice per group. N are indicated on the graphs). (Center panels) Representative immunofluorescence staining of IgG (red), amyloid plaques (thioflavin-S, green) and nuclei (DAPI, blue) from the hippocampus of NTg and 3xTg-AD mice are presented. Statistical analysis: Refer to Table 1. Mann-Whitney test, *P < 0.05; ***P < 0.001; ****P < 0.0001 compared to NTg. b Representative flow cytometry plots for CD138+ bone marrow plasma cells populations