Fig. 2

Knockdown validation by Western blot of the positive hits. Primary mouse microglia were transfected with three individual siRNAs against the indicated target genes. After 3 (b and e) or 4 days (a, c, d, f, g, and h), cells were harvested and subjected to Western blot analysis. Expression of the indicated proteins was normalized to GAPDH expression. Representative Western blots for each protein are depicted below the corresponding graph. Data are mean ± SEM of at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 vs. Scrambled siRNA