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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: The atypical RhoGTPase RhoE/Rnd3 is a key molecule to acquire a neuroprotective phenotype in microglia

Fig. 3

Downregulation of Creb1, RhoE, and p38β reduced BDNF expression in microglia. a Primary microglia were transfected with two individual siRNA oligonucleotides against the indicated target genes. Non-transfected cells (not tr) or cells transfected with Scrambled siRNA were used as controls. After 4 days, gene expression of BDNF was determined by RT-qPCR and normalized to GAPDH expression. Data are expressed as BDNF expression relative to that observed in cells transfected with Scrambled siRNA. b Primary microglia were transfected with siRNA targeting Creb1 (siRNA#2), RhoE (siRNA#1), and p38β (siRNA#2), as representative siRNAs for these genes. Non-transfected cells (not tr) or cells transfected with Scrambled siRNA were used as controls. After 4 days, culture supernatants were collected and used to determine total BDNF protein expression by ELISA. Data are expressed as BDNF protein expression levels relative to that observed in cells transfected with Scrambled siRNA. Data are mean ± SEM of at least three independent experiments.*p < 0.05, **p < 0.01, ***p < 0.001 vs. Scrambled siRNA

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