Skip to main content

Table 1 Validation of siRNA targets resulting in positive differential circularity

From: The atypical RhoGTPase RhoE/Rnd3 is a key molecule to acquire a neuroprotective phenotype in microglia

siRNA Differential circularity P value FDR
Tiam1 #1 2.73 < 10 −16 < 10 −14
Creb1 #2 2.55 < 10 −14 < 10 −11
p38β #3 2.22 < 10 −11 < 10 −10
Tiam1 #2 2.06 < 10 −10 < 10 −9
RhoE #1 1.93 < 10 −9 < 10 −8
Arhgef4 #2 1.84 < 10 −8 < 10 −7
p38β #2 1.79 < 10 −8 < 10 −7
Creb1 #1 1.71 < 10 −7 < 10 −6
Map3k2 #1 1.64 < 10 −6 < 10 −6
Map3k2 #3 1.52 < 10 −6 < 10 −5
IκBα #2 1.46 < 10 −5 < 10 −5
GM-CSFR #1 1.44 < 10 −5 < 10 −5
IκBα #1 1.40 < 10 −5 < 10 −4
Map3k2 #2 1.36 < 10 −5 < 10 −4
Tiam1 #3 1.26 < 10 −4 < 10 −4
p38β #3 1.19 < 10 −4 < 0.01
RhoE #2 1.15 < 0.001 < 0.01
JNK2 #3 1.08 < 0.001 < 0.01
Creb1 #3 1.01 < 0.001 < 0.01
Arhgef4 #1 0.90 < 0.01 < 0.01
GM-CSFR #2 0.83 < 0.01 < 0.01
RhoE #3 0.73 < 0.05 < 0.05
MEK3 #2 0.58 0.05 0.07
Arhgef4 #3 − 0.55 0.06 0.08
GM-CSFR #3 0.42 0.16 0.21
MEK3 #3 − 0.32 0.28 0.35
IκBα #3 0.28 0.34 0.40
MEK3 #1 0.16 0.58 0.64
JNK2 #1 − 0.11 0.70 0.75
JNK2 #2 − 0.08 0.80 0.82
  1. Primary microglia were transfected with three single siRNA oligonucleotides (#1, #2, and #3) per gene and tested for their effect on CM-induced microglia ramification. The change in cell morphology was expressed as differential circularity. Results were sorted by their FDR, and statistically significant genes are marked in italics