Fig. 3

Loss of Par1b/MARK2 alters microglia morphology during brain development in vivo. a Representative 3D reconstructions of microglia from CX3CR1-EGFP-positive Par1b (+/+) and (+/−) mice at P5, P9, P30, and P120 within the parietal association cortex. b Branch complexity was quantified utilizing a Sholl analysis with a somal radius of 10 μm, measuring total lengths of primary, secondary, and tertiary processes. c Morphological aspects were quantified utilizing a Sholl analysis with a somal radius of 10 μm, measuring for number of intersections, average branch length, nodes, and endings. n (animals/microglia) = Par1b (+/+): P5(4/40), P9(4/43), P30(5/41), P120(5/37); Par1b (+/−): P5(4/36), P9(3/39), P30(4/39), P120(3/37), *p < 0.05, **p < 0.01 by type III test of fixed effects using linear mixed model analyses