Fig. 5

Loss of Par1b/MARK2 reduces microglia dynamics in vivo. a Time-lapse Z-stack images were acquired through an open cranial window at the parietal association cortex in anesthetized CX3CR1-GFP-positive Par1b (+/+) and (+/−) mice. Each Z-stack was compressed and made binary. Binary images from each time point (10 total over 20 min) were merged into one final image (merge 0′–20′) to reveal area surveyed by microglia during the imaging timeframe. Scale bar = 10 μm. b The percent area coverage of an individual microglia’s processes was measured. A significant decrease in the percent area surveyed by Par1b (+/−) mice was found when compared to Par1b (+/+) mice, indicating decreased process dynamics. n (animals/microglia) = Par1b (+/+): (6/60); Par1b (+/−): (7/70), ***p < 0.001 by one-way ANOVA