Fig. 9From: Crosstalk between NFκB-dependent astrocytic CXCL1 and neuron CXCR2 plays a role in descending pain facilitationCellular localization of CXCR2 expression in vlPAG after BCP. Immunofluorescence triple staining of CXCR2 (a, f, k), GFAP (g), Neun (l), CD11 (b), and DAPI in vlPAG at day 6 after BCP show that all glial cells and neurons express DAPI + cells (d, i, n). Scale bar in (k): 100 μm. Immunofluorescence triple staining of high-magnification images, indicated in the white boxes of (c, h, m, d, i, n), mainly mainly co-localized with Neun (o), but not with astrocyte (j) and microglia (e). Scale bar in (o): 20 μm. Aq, aqueduct. n = 4Back to article page