Fig. 2

Decreased myelination status in white matter of IUGR newborn piglets. a, b Representative images demonstrating high expression of myelin stained with Luxol fast blue (LFB) in P1 and P4 NG compared with IUGR (d, e) brains (scale bar = 10 mm). The degree of LFB staining was consistent across all white matter (WM) regions in NG brains (b(1–4)), while IUGR displayed overall lower levels with variability between WM regions (e(1–4); scale bar = 500 mm). c IUGR brains presented greater areas with significantly lower LFB staining, as indicated by percentage area for the first quartile. f IUGR displayed a decreased myelin status as shown by the myelin index at both P1 and P4 in IUGR. For c, f IUGR (P1 and P4 n = 6) and NG (P1 n = 5; P4 n = 6). Values are presented as mean ± SEM. Two-way ANOVA with Sidak post-hoc test, significance between IUGR and age-matched NG *p < 0.05; **p < 0.005