Fig. 5

N-[N-(3,5-difluorophenacetyl-l-alanyl)]-S-phenylglycine t-butyl ester (DAPT) inhibited the protein expression of molecules in Notch1 pathway. Western blotting showing DAPT significantly reduced the expression of Notch1, NICD, RBP-Jκ, and Hes-1 (a), with quantitative analysis of Notch1 (b), NICD (c), RBP-Jκ (d), and Hes-1 (e) levels in left hemisphere at 24 h post-SAH; n = 6 in each group; data are expressed as the mean ± SEM. *P < 0.05 versus Sham, ^#P < 0.05 versus SAH + DMSO group. Representative microphotographs of immunofluorescence staining for NICD and Iba1 (f), and immunohistochemistry staining for RBP-Jκ and Hes-1 (g) in the left cerebral cortex at 24 h post-SAH. Scale bar = 50 μm. RBP-Jκ: Recombining binding protein suppressor of hairless; Hes-1: Hes family basic helix loop helix transcription factor 1