Fig. 1

CSF1R antagonism reduces circulating APC populations in uninfected mice. Mice were fed PLX5622 chow or control chow for 2 weeks, and APCs were assessed in blood (a–i) and spleen (j–r). a, j Representative flow cytometry plots of CD11c expression on CD45⁺ cells. b, k Quantification of percentages and c, l total numbers of CD45⁺CD11c⁺ cells. d, m Representative flow cytometry plots of MHCII expression on CD11c⁺CD45⁺. e, n Quantification of percentages and f, o total numbers of MHCII⁺CD11c⁺CD45⁺ cells. g, p Representative flow cytometry plots of CD103 vs CD11b expression on CD11c⁺CD45⁺ cells. h, q Quantification of percentages and i, r total numbers of (I) CD103⁺CD11b^negCD11c⁺CD45⁺, (II) CD103⁺CD11b⁺CD11c⁺CD45⁺, (III) CD103^negCD11b⁺CD11c⁺CD45⁺, and (IV) CD103^negCD11b^negCD11c⁺CD45⁺ cells. For quantification panels, each symbol represents an individual control (black) or PLX5622-treated (red) mouse, and bars indicate mean ± SEM. Data shown represent analysis from one experiment with three to five mice per group, repeated in three independent experiments. Statistical significance was calculated using two-way ANOVA with Sidak’s multiple comparisons test. For all data: ns, not significant at P < 0.05; *P < 0.05; **P < 0.01; ***P < 0.001.

CTRL: Control; PLX: PLX5622