CSF1R antagonism reduces circulating APC populations in uninfected mice. Mice were fed PLX5622 chow or control chow for 2 weeks, and APCs were assessed in blood (a–i) and spleen (j–r). a, j Representative flow cytometry plots of CD11c expression on CD45+ cells. b, k Quantification of percentages and c, l total numbers of CD45+CD11c+ cells. d, m Representative flow cytometry plots of MHCII expression on CD11c+CD45+. e, n Quantification of percentages and f, o total numbers of MHCII+CD11c+CD45+ cells. g, p Representative flow cytometry plots of CD103 vs CD11b expression on CD11c+CD45+ cells. h, q Quantification of percentages and i, r total numbers of (I) CD103+CD11bnegCD11c+CD45+, (II) CD103+CD11b+CD11c+CD45+, (III) CD103negCD11b+CD11c+CD45+, and (IV) CD103negCD11bnegCD11c+CD45+ cells. For quantification panels, each symbol represents an individual control (black) or PLX5622-treated (red) mouse, and bars indicate mean ± SEM. Data shown represent analysis from one experiment with three to five mice per group, repeated in three independent experiments. Statistical significance was calculated using two-way ANOVA with Sidak’s multiple comparisons test. For all data: ns, not significant at P < 0.05; *P < 0.05; **P < 0.01; ***P < 0.001.
CTRL: Control; PLX: PLX5622