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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: CSF1R antagonism limits local restimulation of antiviral CD8+ T cells during viral encephalitis

Fig. 2

PLX5622-treated mice exhibit increased mortality, increased encephalitis score, and impaired virologic control compared with control mice following peripheral infection with WNV-NY. ad Mice were fed chow containing PLX5622 or control chow for 2 weeks, then infected with 102 or 104 PFU via footpad infection, then monitored for (a) mortality, (b) weight loss, and (c, d) encephalitis score for up to 25 dpi. a Survival curves show a significant increase in mortality in mice treated with PLX5622 compared with control-treated mice infected with either viral inoculum dose, as calculated by log-rank (Mantel-Cox) test. b Weight was measured daily during acute illness as a measure of illness. After death, the last measured weight was carried through to the end of the experiment. Significantly greater weight loss was measured in PLX5622-treated mice compared with controls infected with 102 PFU as calculated by two-way ANOVA with matched values comparing group means without multiple comparisons. c Clinical scores of PLX5622 or control-treated mice infected with 102 PFU or d 104 PFU as indicated during acute illness. 0 = subclinical, 1 = hunched/ruffled fur, 2 = altered gate/slow movement, 3 = no movement, but responsive to stimuli, 4 = moribund, 5 = dead. ek Tissue viral loads as measured by plaque assay at 2, 4, 6, and 8 dpi following infection with 102 PFU in control (black) or PLX5622-treated (red) mice. l Serum viral loads as measured by qRT-PCR. el Data are presented as scatter plots with each mouse represented by a dot with the SEM indicated by a line. Dotted lines in el indicate assay limit of detection. All data presented are the compilation of two independent experiments with 10 mice per group. Statistical significance was calculated using two-way ANOVA with Sidak’s multiple comparisons test. For all data: ns, not significant at P < 0.05; *P < 0.05; **P < 0.01; ****P < 0.0001

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