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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: CSF1R antagonism limits local restimulation of antiviral CD8+ T cells during viral encephalitis

Fig. 4

CSF1R antagonism reduces response of APCs in peripheral immune compartments following peripheral infection with WNV-NY. Mice were fed PLX5622 or control chow for 2 weeks, then infected via f.p. with WNV-NY (100 PFU). At 4 dpi, leukocytes were isolated from blood (aj) and draining popliteal LNs (k–t). a, k Representative flow cytometry plots of CD11c expression on CD45+-gated cells and b, l fluorescence minus one (FMO) gating controls. c, m Quantification of percentages and d, n total numbers of CD11c+ CD45+ cells. e, o Representative flow cytometry plots of MHCII+ expression on CD11c+CD45+ cells. f, p Quantification of percentages and g, q total numbers of MHCII+CD11c+CD45+ cells. h Representative flow cytometry plots of CD86+ expression on CD11c+CD45+ cells. i Quantification of percentages and j total numbers of CD86+CD11c+CD45+ cells. r Representative flow cytometry plots of CD80+ expression on CD11c+CD45+ cells. s Quantification of percentages and t total numbers of CD80+CD11c+CD45+ cells. For quantification panels, each symbol represents an individual control- (black) or PLX5622-treated (red) mouse, and bars indicate mean ± SEM. Data shown represent analysis from one experiment with three to five mice per group. Statistical significance was calculated using unpaired t test in all panels except d and n, which used two-way ANOVA with Sidak’s multiple comparisons test. For all data: ns, not significant at P < 0.05; *P < 0.05; **P < 0.01

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