Fig. 5

CSF1R antagonism reduces co-stimulatory signal 2 on splenic APCs during i.c. infection with WNV-NS5-E218A. Mice were fed PLX5622 chow or control chow for 2 weeks, then infected i.c. with WNV-NS5-E218A (10⁴ PFU). At 8 dpi, splenic leukocytes were analyzed. a Representative flow cytometry contour plots of CD11c and MHCII expression on CD45⁺-gated cells and b quantification of percentages and c total numbers of CD11c⁺MHCII⁺CD45⁺ cells. d Representative flow cytometry plots of CD80 expression on CD11c⁺MHCII⁺CD45⁺ cells. e Quantification of percentages and f total numbers of CD80⁺CD11c⁺MHCII⁺CD45⁺ cells. g Representative flow cytometry plots of CD86 expression on CD11c⁺MHCII⁺CD45⁺ cells. h Quantification of percentages and i total numbers of CD86⁺CD11c⁺MHCII⁺CD45⁺ cells. j Representative flow cytometry histograms of CD80 expression on CD11c⁺MHCII⁺CD45⁺ and k quantification of MFI. l Representative flow cytometry histograms of CD86 expression on CD11c⁺MHCII⁺CD45⁺ and m quantification of MFI. AU, arbitrary units. For quantification panels, each symbol represents an individual control- (black) or PLX5622-treated (red) mouse, and bars indicate mean ± SEM. Data represent analysis from one experiment with three to four mice per group. Statistical significance was calculated using unpaired two-way t tests. For all data: ns, not significant at P < 0.05; *P < 0.05; **P < 0.01